Is it possible to mix tapes if we have an LTO3 drive and an LTO5 drive installed on a TL4000? Could we separate by creating a second logical drive?

Is it possible to mix tapes if we have an LTO3 drive and an LTO5 drive installed on a TL4000?  Could we separate by creating a second logical drive?  

We have a TL4000 with two LTO5 fibre drives and two LTO3 SAS drives.  I am not certain this arrangement will work.  Has anybody here done anything similar?  

Thanks,

Mike